By Leland G. Johnson
A gentle progress in enrollments in developmental biology classes has caused WCB/McGraw-Hill to contemplate the way to supply a laboratory handbook that addresses the troubles of an ever-growing variety of educators and scholars. Many features distinguish this guide from others out there. styles and Experiments in Developmental Biology integrates a descriptive learn of developmental styles with experimental research of developmental procedures.
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Additional resources for Johnson & Volpe's patterns & experiments in developmental biology
Echinoderm Development Suggestions for Further Investigation of Echinoderm Development © The McGraw−Hill Companies, 2004 The process of development takes place in a series of stages that occur in a regular sequence. Cleavage of the fertilized egg leads to the blastula stage (stages 8–9), which is followed by the process of gastrulation (stages 10–12). Gastrulation is a complex set of cell movements that results in the establishment and positioning of the three germ layers (ectoderm, endoderm, and mesoderm).
The cell movements during gastrulation were first investigated using vital stains that color, but do not damage, the embryonic cells. In a series of experiments in the 1920s,Vogt briefly placed small agar chips saturated with Nile Blue sulphate against various areas of amphibian embryos at the beginning of gastrulation. He then traced the movement of the stained cells and determined their eventual position Laboratory 5 47 Johnson:Johnson & Volpe’s Patterns & Experiments in Developmental Biology, 3/e II.
Amphibian Development © The McGraw−Hill Companies, 2004 4. 3 A fate map of an early gastrula stage frog embryo, based on Vogt’s experiments with vital stains. 48 Laboratory 5 Johnson:Johnson & Volpe’s Patterns & Experiments in Developmental Biology, 3/e II. Amphibian Development 4. This permitted construction of fate maps of the early gastrula, showing the eventual roles of cells in various regions (fig. 3). There are, however, variations among amphibian species in the details of fate maps. For example, fate maps of the clawed frog (Xenopus laevis) show that the fates of cells in some areas of the early gastrula depend upon whether these cells are at the surface or lie in deeper layers.